When looking at cells with a microscope, the length of different stages of the cell cycle can be estimated using the formula: \(\text{Length of time in phase}=\) \(\frac{\text{observed number of ...

Figure 1: Specific cell associations that define the stages of the cycle of the seminiferous epithelium ... under a transilluminating dissection microscope (Supplementary Figure 1 online).

The stain can be applied to live, detergent-permeabilized or fixed cells for quantification of their distribution among the three main phases of the cell cycle (G 0 /G 1, S and G 2 /M).