This technique is very common in biological and chemical research. Purdue University has numerous HPLC units located in several laboratories around campus. Because organic solvents (e.g., methanol and ...

The HPLC used in this lab has a column that has been carefully packed with C-18 particles, which are less than 10 micrometers in diameter. Since the particles used in the column are so small, it is ...

Reversed phase HPLC columns are the inverse of normal phase chromatography. The stationary bed is non-polar (hydrophobic) in nature, while the mobile phase is a polar liquid, such as mixtures of water ...