Analysis of the brain palmitoyl-proteome using both acyl-biotin ...
2017年6月12日 · To address this issue, we used both acyl-biotin exchange and acyl-resin-assisted capture approaches using rat brain as a common protein source.
Palmitoylated proteins: purification and identification
This proteomic protocol purifies and identifies palmitoylated proteins (i.e., S-acylated proteins) from complex protein extracts. The method relies on an acyl-biotinyl exchange chemistry in which biotin moieties are substituted for the thioester-linked protein acyl-modifications through a …
Palmitoylated proteins: purification and identification
2007年6月21日 · A protein extract derived from total yeast membranes was split in half and purified through the +HA and −HA acyl-biotinyl exchange (ABE) protocols in parallel.
The chemical toolbox for monitoring protein fatty acylation …
2010年6月17日 · Acyl-biotin exchange. Acyl-biotin exchange (ABE) is a nonradioactive method that was developed in 2004 by the Green group for detecting protein palmitoylation 49 .
Detection of protein palmitoylation in cultured hippocampal …
2013年2月18日 · Development of the Acyl-Biotin Exchange (ABE) assay enables more rapid and high sensitivity detection of palmitoylated proteins, and is optimal for measuring the dynamic turnover of palmitate on neuronal proteins.
Low-Background Acyl-Biotinyl Exchange Largely Eliminates the ...
2019年8月8日 · Acyl-biotinyl exchange (ABE), a widely used method for the enrichment of S-acylated proteins, has the potential of capturing the entire S-acylproteome in any type of biological sample.
A Simple, Semi-Quantitative Acyl Biotin Exchange-Based Method …
2023年3月21日 · The aim of this research is to provide a straightforward and sensitive procedure to detect protein palmitoylation based on Acyl Biotin Exchange (ABE) chemistry. Our protocol setup consists of co-immunoprecipitation of native proteins (i.e., CD63), followed by the direct detection of palmitoylation on proteins immobilized on polyvinylidene ...
Protocol for a semi-quantitative approach to identify protein S ...
2024年6月21日 · We describe steps for initial blocking of free thiols in protein lysates, subsequent replacement of thioester-linked palmitate groups with a biotin tag for affinity enrichment, and identification of palmitoylated proteins by SDS-PAGE.
Global, site-specific analysis of neuronal protein S-acylation
2017年7月5日 · We have developed a workflow that builds upon standard acyl-biotin exchange assays to permit large scale analysis of palmitoylation sites.
Low-Background Acyl-Biotinyl Exchange Largely Eliminates the ... - PubMed
2019年8月6日 · Acyl-biotinyl exchange (ABE), a widely used method for the enrichment of S-acylated proteins, has the potential of capturing the entire S-acylproteome in any type of biological sample.