Tubes coated with an anticoagulant (e.g., EDTA, heparin, or sodium citrate) are used for plasma isolation. Immediately after blood collection, the tubes are inverted several times to ensure proper mixing of anticoagulant with whole blood. Since K2-EDTA is best at preserving cellular components (e.g., DNA) and morphology of blood cells at

Pipette a minimum of 1mL plasma from the lavender top (EDTA) tube into each pre-labeled aliquot vial. Depending on the hematocrit of the patient, one 10 mL tube of blood yields 3 to 5 aliquots of plasma in volumes of 1 mL per vials.

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Whole blood will be aliquoted into sterile tubes upon receipt by carefully inverting the blood tube so that it is gently mixed before pipetting appropriate amounts (protocol specific) of whole blood into appropriate storage tubes using clean pipette tipps between