Overview of Protein Labeling | Thermo Fisher Scientific - US
Labeling strategies result in the covalent attachment of different molecules, including biotin, reporter enzymes, fluorophores, and radioactive isotopes, to the target protein or nucleotide sequence. While multiple types of labels are available, …
Principles of protein labeling techniques - PubMed
The major purposes for protein labeling are monitoring of biological processes, reliable quantification of compounds and specific detection of protein modifications and isoforms in multiplexed samples, enhancement of detection sensitivity, and simplification of …
Labeling proteins on live mammalian cells using click chemistry
2015年4月23日 · We provide a detailed protocol for using commercially available ncAAs and dyes for labeling the insulin receptor, and we discuss the optimal surface-labeling conditions and the limitations of...
Chemical tags and beyond: Live-cell protein labeling ...
2023年8月28日 · We focus on advances in live-cell labeling strategies and fluorescent probes, especially the HaloTag, SNAP-tag, TMP-tag, and unnatural amino acid systems and their applications.
Protein Labeling - NEB
The SNAP-tag ®, CLIP-tag™, and ACP-tag/MCP-tag protein labeling technologies offer an innovative alternative to traditional fluorescent proteins for studying the function and localization of proteins in living and fixed cells.
Site-Specific Protein Labeling with SNAP-Tags - PMC
Site-specific labeling of cellular proteins with chemical probes is a powerful tool for studying proteins function in living cells. A number of small peptide and protein tags have been developed that can be labeled with synthetic probes with high ...
Imaging and manipulating proteins in live cells through ...
2015年11月17日 · Protein labeling has been used to both visualize and manipulate proteins in living cells and has helped to provide insights into various biological questions, ranging from drug target...
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